LOCUS KO-first_condition_ready_80275_MGI:1925836 23892 bp dna circular UNK
DEFINITION Mus musculus targeted KO-first, conditional ready, lacZ-tagged
mutant vector Srbd1 targeting project(s): 80275
ACCESSION unknown
SOURCE Mus musculus
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT cassette: L1L2_Bact_P
COMMENT design_id: 212874
COMMENT backbone: R3R4_pBR_DTA+_Bsd_amp
FEATURES Location/Qualifiers
misc_feature 29..152
/note="R4 gateway site"
promoter 399..911
/note="PGK promoter"
CDS 925..1581
/note="DTA"
misc_feature 1706..1959
/note="BGH pA"
promoter 2034..2100
/note="EM7 promoter"
CDS 2101..2499
/note="BsdR"
CDS complement(4193..5053)
/note="AmpR"
misc_feature 5272..5395
/note="R3 gateway site"
misc_feature 5401..5408
/note="AsiSI"
misc_feature 1..5433
/note="Synthetic Backbone"
exon 8627..8811
/db_xref="ENSEMBL:ENSMUSE00000375453"
/rank="20"
/note="ENSMUSE00000375453"
misc_feature 5434..11293
/note="5 arm"
misc_feature 11321..11345
/note="B1 Gateway"
misc_feature 11358..11405
/note="Frt"
intron 11406..12280
/note="mouse En2 intron"
misc_feature 12278..12280
/note="Splice Acceptor"
exon 12281..12453
/note="mouse En2 exon"
misc_feature 12465..13058
/note="ECMV IRES"
gene 13059..16119
/gene="lacZ"
/note="lacZ"
misc_feature 16158..16401
/note="SV40 polyadenylation site"
misc_feature 16405..16438
/note="loxP"
promoter 16465..16984
/note="human beta actin promoter"
gene 17010..17804
/gene="NeoR"
/note="NeoR"
misc_feature 18004..18245
/note="SV40 polyadenylation site"
misc_feature 18262..18309
/note="Frt"
misc_feature 18316..18349
/note="loxP"
misc_feature 12295..12372
/note="FCHK std_gpr"
misc_feature 18365..18385
/note="B2 Gateway"
misc_feature 11294..18415
/note="Synthetic Cassette"
exon 18753..19571
/db_xref="ENSEMBL:ENSMUSE00000605979"
/type="targeted"
/rank="21"
/note="target exon 1 ENSMUSE00000605979"
misc_feature 18416..19707
/note="Critical Region"
misc_feature 19731..19764
/note="Downstream LoxP"
misc_feature 19708..19787
/note="synthetic loxP region"
misc_feature 19788..23892
/note="3 arm"
ORIGIN
1 ccactggccg tcgttttaca ttaattaaca actttgtata gaaaagttga acgagaaacg
61 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
121 tactgtaaaa cacaacatat ccagtcacta tggcggccgc aatcgggcag cgttgggtcc
181 tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg ctggcggggt
241 tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc
301 tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa
361 agtctggaaa cgggccgcct agcgatagct gtttatcgaa ttctaccggg taggggaggc
421 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
481 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
541 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
601 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
661 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
721 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
781 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
841 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
901 cctttcgacc tgcaggtcct cgccatggat cctgatgatg ttgttgattc ttctaaatct
961 tttgtgatgg aaaacttttc ttcgtaccac gggactaaac ctggttatgt agattccatt
1021 caaaaaggta tacaaaagcc aaaatctggt acacaaggaa attatgacga tgattggaaa
1081 gggttttata gtaccgacaa taaatacgac gctgcgggat actctgtaga taatgaaaac
1141 ccgctctctg gaaaagctgg aggcgtggtc aaagtgacgt atccaggact gacgaaggtt
1201 ctcgcactaa aagtggataa tgccgaaact attaagaaag agttaggttt aagtctcact
1261 gaaccgttga tggagcaagt cggaacggaa gagtttatca aaaggttcgg tgatggtgct
1321 tcgcgtgtag tgctcagcct tcccttcgct gaggggagtt ctagcgttga atatattaat
1381 aactgggaac aggcgaaagc gttaagcgta gaacttgaga ttaattttga aacccgtgga
1441 aaacgtggcc aagatgcgat gtatgagtat atggctcaag cctgtgcagg aaatcgtgtc
1501 aggcgatctc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc
1561 tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact
1621 actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca
1681 gtggtggaat gcagatccta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
1741 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
1801 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
1861 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
1921 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgag
1981 atccactagt tctagcctcg aggctagagc ggccgagttt atcctcgagc acgtgttgac
2041 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
2101 atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc
2161 aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc
2221 cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg
2281 gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga
2341 aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg
2401 catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt
2461 cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag cacttcgtgg ccgaggagca
2521 ggactgagaa ttccccggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
2581 ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
2641 ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
2701 cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
2761 ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc
2821 aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
2881 ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
2941 gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
3001 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
3061 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
3121 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
3181 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
3241 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
3301 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
3361 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
3421 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
3481 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
3541 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
3601 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
3661 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
3721 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
3781 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
3841 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
3901 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
3961 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
4021 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
4081 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
4141 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
4201 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
4261 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
4321 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
4381 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
4441 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
4501 ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
4561 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
4621 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
4681 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
4741 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
4801 cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
4861 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
4921 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
4981 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
5041 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
5101 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
5161 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
5221 ataaaaatag gcgtatcacg aggccctttc gtcttcaaga aaattgtcga ccatagtgac
5281 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
5341 atattgatat ttatatcatt ttacgtttct cgttcaactt tattatacaa agttgcggac
5401 gcgatcgccg gcggataaca atttcacaca ggatcctttc atttccaaaa tagctgtgct
5461 cttgtattta attttttact tgttgacgaa atctctatag aatgaacgga tagagtgtga
5521 cagctatggc agtctctgag gacagtggtc tttgcggtgg taacatggct ctctccatgg
5581 gctatacacc atttgtgctc tttgtaaagt gatggtgtta tatcacctag gttctgagct
5641 ctgcagggtg tcatgttgct ctatgaaaca aacccctcag cttctaaatc cctgtgctgg
5701 aaatgattac ataaatctga atggccattt ccagggtctt acgaatctgg agaattcctt
5761 actctttggg ggaagagttt tacaaagact ttttctttgt ggagaaggac ctggcctttg
5821 tagcaagctt ctgttgaaac cgtccgcagt gtcagcttgg gtagctttgc attgaaaagt
5881 aagcatgatg tgtgctactc tgtgctctaa ctccgtgcag atgtgtgcta agagaacatg
5941 atgtgctcat ttgttgcatt tatgctactt tcttacttta agtaagatgg atgaaattac
6001 atattaaata aaaacagcag ctccaacagc agcccttagc aaatggaaaa gaccttccag
6061 agacagtaga gatggcttta ataactcatt gactcgagag aactgtctat ttaaaagctt
6121 gtgcataata acttctttgt gggattttct tggtgctaac tgtctaatat tcagaagtga
6181 tctgccatta aattgtgttg gcacagtatt gcccaacaca tcggtcttct gtcttggcca
6241 aatataacag taattttcta tatgcactca gtgacatagt acatgatacg aaactccatg
6301 gagcagcggt agtgcatggc ctgggcagcc tgatagtcct gcattttaaa gttcctgtta
6361 gaagtgagtg aaacctacag ctagcccagc atgctgtgtg tttgtcatta tgtgtttatt
6421 ctgggctgca agctccttac atgtgcactt tcccgtggca ctttggagct aggaagttct
6481 ttgtgttggg cacacttctc actgccagag agaactttag actgtagagg agacgttccc
6541 tgtgctactg ccaaagccag gtctgatgag tggctccagc atgaagcatc tcgtcccatc
6601 ctgggctgcc gagtatgtct gagacccacc ccagggctgg ctctgcttct tcccagtttc
6661 tcccccttct gttggctttt tctgtgacct ggttaacttt aagaaagttt tcaaaatgca
6721 aaaactactt tgttccaaaa tagggttgtt taaaaatagg gttgatcaat agaaagcatt
6781 agctggaggc caacatggca cttccttatt gatctcagca gctgctgccc acaggcaggc
6841 ttctgtgagg gtgaaaggat atatgaactg cactcccagg acaaacagaa agcagacttc
6901 ctttttatat ggaagagatt ctgcagtaca cagctatcca ctggacggag tacaggggcc
6961 ccagtgaagg agctaaagaa aggacccaag gagttgaagg ggtttacagc tccataggag
7021 gaacaacaat atgcaccaac cagtaccctc agagctttca gggactaaac caccaagcaa
7081 agaatacaca tggtagactc atggctcaca gcagcagagg atggcctagt cggtcatcaa
7141 tgagaggaga ggcccttggt ccaatgaagg ctctctctat gcccagtgta ggggaaatcc
7201 agggccagga agtgggagtg gatgggttgg tgagcagggg gaagggaggg gagggggagg
7261 ggggctttgg aaggagacca ggaaagggga taacatttca aatgtaaata aagaaaatat
7321 ctaataaaat aaaaaatgga aaaacaaaaa gaaaaaaagt gtaaagtgga aaaaaaatat
7381 gactctggaa atgtagaagg cttgcacatt tgaatccaag agattcccta gttaaatgtg
7441 aggtgaaagg cctttctcac caggcttgag gttatcagcc ctggatgtca ctgaagaggg
7501 ctgctctgcc tgagcatggt gcccagaaag attaggagtg gcccgcagtc cgtcctgcac
7561 cgtgcagttc ctgccacggg ggctgtaggc agagacacac tccagcagcc tccttttccc
7621 ttccggtatt gagagacatt ctcagaggaa agactgaggg catcaggatc tgtttgatta
7681 ctgtgttgac atttttaaag tgccattaat cataggaaat agaacttaat gatgtagaaa
7741 aatacccagc cagagaatgt caggaaagag agccatggcc cggaactcac atgaaactgg
7801 agcaaactcg gtcttacccg taacttgttg attcgtgttg tcatggtttt acctcattaa
7861 ctgggcagtg tctgtgcaag ccaggaaggc agcaggcagg gcgaaggtga ggtccaaggc
7921 agcatcgctg ctcagtggat gtagtggatg gaaggacatt cagcttgcat tgcagctttg
7981 ggggtttact tgcattcttt tgggggggca tcactttttc tttcacatcc ctccctttta
8041 tactgttgtg ttgctgtggg gagcgtgagc acacccaccc ctaaccgccc tctgtgattt
8101 actttcccgc tgaatactcc gagtgttttt taggtaattt cacttgtgtg tggctcgatg
8161 gcatttgtgt caccctccca atcacaggcc acaggcgtag ctattcatgt acaatggaaa
8221 ttgagcagtg tcaaaagcga ggagtgcttg acacatgtca tgttggactc tggtatcatc
8281 aatggatcat gtttgtctat taatctaaat tcaaatgttt tcctactgaa aatttcagga
8341 aacgtgagat aatatagaca gccttgtcta ggaactacac atgtgacact gaactgtcat
8401 gaccagcaca ctgtaaagtg ttccttgcca cacttggaaa atagcacaag agcctaaacc
8461 ccccttctcc ccttgcccac atgcctttag ctaaatctta acacagcaca gagtatactg
8521 tagactctca actgactgat gtgcaagtgt cctgggatga gagaatgtga aggtgaccag
8581 aaggtaagat cactgataaa ttgttgggct tttcttccca ctgaaggttt ttatcgttca
8641 ttggggggac aatgtgtgag attggaaagc ctgaaatgca gcagaaaata aacgtgtctc
8701 tcgggaaaga aggcatagag gaaactgctg aaaggttaca gacaactgtg cacacactgc
8761 aggtcatcat agatggactg agccagccca aaacctttga catccgaaca ggtgagttgt
8821 cgcccttgtc tccaggaagt tcccatcgcc atcttaggtc ctctcgacag gagagggcca
8881 caggagaagg ctgagagtca gcatccgtcg tccacaggag agggccaagg gtcagcgttc
8941 atcctccgca ctcacttaga ttcctgtctg gagccaaagc tacaagtatg gcttcggcct
9001 cctggtccat gttgctgtgc tgctgcacca agctggacac ctctcatgtg ctgtgcttct
9061 gtgtgcatgg aggcatgctc tccctgagtc tcctgtaaac gtctttgtgt ctgaagagcc
9121 aggagggaaa gctttccatt cacaccaaaa agtgaatctt cttcctaaat tacagccgtc
9181 caggtttctg gattccattt cactgagcac attgaacaaa taagatgata tctctgtgct
9241 gattgtcttt ctaatttcga gtgttctcag ctttctgtgc agggagaggg taaaggaagt
9301 gtaatagccg tggttgttga gagcaatgac ccattgagac agaattgcag ctggagaaaa
9361 caaaacactg agtaagacca gtgccaaaag tgggcatcaa agcctgcctg ttctcataga
9421 aagttgctgc tcctaaatac gggactgtgg agaagcctgc ttttcccctt gtggactcca
9481 gggctgtctc ttgcagggac actaagacca gtgtgtctca tgaggggtgg gattggtaat
9541 atgcctatac actcactgga tggttgacag gggcacttga gctataggta gcctcgactc
9601 tattttcctg tccatcttta tttttctatc aaggtgtcga ttgctaaata tctcagttac
9661 atgagggact caaagcaaag gcaagcactc agttagccca gctagacaga gcccctcctt
9721 gaggagcctc tagttgagca gcatgcggat gggcctgggc tctccagagg gcattccaaa
9781 caggcctctc caagcacact gtcaacacat ttccagggga gaggcagatg actttggagg
9841 tggggggcag ggtagacagt attttgggta tttccatgtt acagtatttg tattacatct
9901 cttttctcct tttctcaaaa gtgtttcctg tcttatgcct tattcctccc tttcactcat
9961 ctggcttctg ctctttgctg acatcgttcc tgttgtttaa gccctccttg ctggttagtt
10021 tccttaattc agagcacact ggtgccgtgg cgtgactgct cccatggccc ttgatgtgta
10081 agtgtacatg gttcatggag gctttctgct ttctccgtgc tcccagctgt cttggggaac
10141 ctgctgagtg gttcctgact ggttttcatg ccaagctaaa ctgagcagct gatgagaacc
10201 ccatggggat tggcagccac tggattctac aaggcaagga ggcgagagat ttctaagcat
10261 gaaactgcct tactggcctg gggaccagcc tacctctgcc ctgggcctta gtttcacttc
10321 atgttgtcat ggtctcaacc ttgtgtggtt caaattacca gaagttcctg ctttggaatt
10381 tagcatcagg tgaaatccca gcgtccactc ccaggttggt gtgtttaatg aggacagtga
10441 ggtgtagggt ttatggattt ttgtcttgtt ttgcagaagt ggaaggagca cagtttctaa
10501 aaaagatcgt ggacttaagg tggtttgact ggggagggaa aggaagaggt gaccagttgt
10561 taatacaaat gtaaaagata gctccacaag taatcagggt aaagccaaca gctgtggggg
10621 aaaaggaact gggtcagagg gttggagtgg gggcaggggg atgtctaagt aaaggagatc
10681 cagctagaat gtgttgtttg ttatcagaat caacactcag tgatcctagg gaagggagct
10741 gagctggagg ggtaaatgca ggctgtgggg actcacaagc cccatttaaa cttcttgtct
10801 cactatctca cacctgtgtt gttctatgtt atctggagcc atagtggcac gtagcctgct
10861 ttgtgttcat gttttagggg ctactgtcag tgaatagaga tatgtagact gggatgggcc
10921 aagaaagggc tgaaaagagc ctaagacggg gacctagggt cttcgccatt gtgtatctgg
10981 catacagcag agcaccagcc tgaggtaatg atagtaatcg gtcaatcaac tgaactggag
11041 gaagagggag gctagctctg gcagactggt tggtgtgcat tctgagccat aggtaattgg
11101 gaacctatac cttctggttg aataaatgtg tttgaaatag aattgattcc tgaatactta
11161 ctaaagtgaa gagagaacat ttgacttggc tcacatttat tcaaaagcta gtggaacgtt
11221 gaccaccttg aaacaattta agaatccagg cactgaaagc caactccccc tcttgtgtct
11281 ccagggctcc cccaaggcgc ataacgatac cacgatatca acaagtttgt acaaaaaagc
11341 aggctggcgc cggaaccgaa gttcctattc cgaagttcct attctctaga aagtatagga
11401 acttcgaacc ctttcccaca ccaccctcca cacttgcccc aaacactgcc aactatgtag
11461 gaggaagggg ttgggactaa cagaagaacc cgttgtgggg aagctgttgg gagggtcact
11521 ttatgttctt gcccaaggtc agttgggtgg cctgcttctg atgaggtggt cccaaggtct
11581 ggggtagaag gtgagaggga caggccacca aggtcagccc ccccccccta tcccatagga
11641 gccaggtccc tctcctggac aggaagactg aaggggagat gccagagact cagtgaagcc
11701 tggggtaccc tattggagtc cttcaaggaa acaaacttgg cctcaccagg cctcagcctt
11761 ggctcctcct gggaactcta ctgcccttgg gatccccttg tagttgtggg ttacatagga
11821 agggggacgg gattcccctt gactggctag cctactcttt tcttcagtct tctccatctc
11881 ctctcacctg tctctcgacc ctttccctag gatagacttg gaaaaagata aggggagaaa
11941 acaaatgcaa acgaggccag aaagattttg gctgggcatt ccttccgcta gcttttattg
12001 ggatccccta gtttgtgata ggccttttag ctacatctgc caatccatct cattttcaca
12061 cacacacaca ccactttcct tctggtcagt gggcacatgt ccagcctcaa gtttatatca
12121 ccacccccaa tgcccaacac ttgtatggcc ttgggcgggt catccccccc cccaccccca
12181 gtatctgcaa cctcaagcta gcttgggtgc gttggttgtg gataagtagc tagactccag
12241 caaccagtaa cctctgccct ttctcctcca tgacaaccag gtcccaggtc ccgaaaacca
12301 aagaagaaga accctaacaa agaggacaag cggcctcgca cagccttcac tgctgagcag
12361 ctccagaggc tcaaggctga gtttcagacc aacaggtacc tgacagagca gcggcgccag
12421 agtctggcac aggagctcgg tacccggaag atctggactc tagagaattc cgcccctctc
12481 cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
12541 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
12601 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
12661 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
12721 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
12781 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
12841 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
12901 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct
12961 ttacatgtgt ttagtcgagg ttaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
13021 tttcctttga aaaacacgat gataagcttg ccacaaccat ggaagatccc gtcgttttac
13081 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
13141 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
13201 gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc accagaagcg gtgccggaaa
13261 gctggctgga gtgcgatctt cctgaggccg atactgtcgt cgtcccctca aactggcaga
13321 tgcacggtta cgatgcgccc atctacacca acgtgaccta tcccattacg gtcaatccgc
13381 cgtttgttcc cacggagaat ccgacgggtt gttactcgct cacatttaat gttgatgaaa
13441 gctggctaca ggaaggccag acgcgaatta tttttgatgg cgttaactcg gcgtttcatc
13501 tgtggtgcaa cgggcgctgg gtcggttacg gccaggacag tcgtttgccg tctgaatttg
13561 acctgagcgc atttttacgc gccggagaaa accgcctcgc ggtgatggtg ctgcgctgga
13621 gtgacggcag ttatctggaa gatcaggata tgtggcggat gagcggcatt ttccgtgacg
13681 tctcgttgct gcataaaccg actacacaaa tcagcgattt ccatgttgcc actcgcttta
13741 atgatgattt cagccgcgct gtactggagg ctgaagttca gatgtgcggc gagttgcgtg
13801 actacctacg ggtaacagtt tctttatggc agggtgaaac gcaggtcgcc agcggcaccg
13861 cgcctttcgg cggtgaaatt atcgatgagc gtggtggtta tgccgatcgc gtcacactac
13921 gtctgaacgt cgaaaacccg aaactgtgga gcgccgaaat cccgaatctc tatcgtgcgg
13981 tggttgaact gcacaccgcc gacggcacgc tgattgaagc agaagcctgc gatgtcggtt
14041 tccgcgaggt gcggattgaa aatggtctgc tgctgctgaa cggcaagccg ttgctgattc
14101 gaggcgttaa ccgtcacgag catcatcctc tgcatggtca ggtcatggat gagcagacga
14161 tggtgcagga tatcctgctg atgaagcaga acaactttaa cgccgtgcgc tgttcgcatt
14221 atccgaacca tccgctgtgg tacacgctgt gcgaccgcta cggcctgtat gtggtggatg
14281 aagccaatat tgaaacccac ggcatggtgc caatgaatcg tctgaccgat gatccgcgct
14341 ggctaccggc gatgagcgaa cgcgtaacgc gaatggtgca gcgcgatcgt aatcacccga
14401 gtgtgatcat ctggtcgctg gggaatgaat caggccacgg cgctaatcac gacgcgctgt
14461 atcgctggat caaatctgtc gatccttccc gcccggtgca gtatgaaggc ggcggagccg
14521 acaccacggc caccgatatt atttgcccga tgtacgcgcg cgtggatgaa gaccagccct
14581 tcccggctgt gccgaaatgg tccatcaaaa aatggctttc gctacctgga gagacgcgcc
14641 cgctgatcct ttgcgaatac gcccacgcga tgggtaacag tcttggcggt ttcgctaaat
14701 actggcaggc gtttcgtcag tatccccgtt tacagggcgg cttcgtctgg gactgggtgg
14761 atcagtcgct gattaaatat gatgaaaacg gcaacccgtg gtcggcttac ggcggtgatt
14821 ttggcgatac gccgaacgat cgccagttct gtatgaacgg tctggtcttt gccgaccgca
14881 cgccgcatcc agcgctgacg gaagcaaaac accagcagca gtttttccag ttccgtttat
14941 ccgggcaaac catcgaagtg accagcgaat acctgttccg tcatagcgat aacgagctcc
15001 tgcactggat ggtggcgctg gatggtaagc cgctggcaag cggtgaagtg cctctggatg
15061 tcgctccaca aggtaaacag ttgattgaac tgcctgaact accgcagccg gagagcgccg
15121 ggcaactctg gctcacagta cgcgtagtgc aaccgaacgc gaccgcatgg tcagaagccg
15181 ggcacatcag cgcctggcag cagtggcgtc tggcggaaaa cctcagtgtg acgctccccg
15241 ccgcgtccca cgccatcccg catctgacca ccagcgaaat ggatttttgc atcgagctgg
15301 gtaataagcg ttggcaattt aaccgccagt caggctttct ttcacagatg tggattggcg
15361 ataaaaaaca actgctgacg ccgctgcgcg atcagttcac ccgtgcaccg ctggataacg
15421 acattggcgt aagtgaagcg acccgcattg accctaacgc ctgggtcgaa cgctggaagg
15481 cggcgggcca ttaccaggcc gaagcagcgt tgttgcagtg cacggcagat acacttgctg
15541 atgcggtgct gattacgacc gctcacgcgt ggcagcatca ggggaaaacc ttatttatca
15601 gccggaaaac ctaccggatt gatggtagtg gtcaaatggc gattaccgtt gatgttgaag
15661 tggcgagcga tacaccgcat ccggcgcgga ttggcctgaa ctgccagctg gcgcaggtag
15721 cagagcgggt aaactggctc ggattagggc cgcaagaaaa ctatcccgac cgccttactg
15781 ccgcctgttt tgaccgctgg gatctgccat tgtcagacat gtataccccg tacgtcttcc
15841 cgagcgaaaa cggtctgcgc tgcgggacgc gcgaattgaa ttatggccca caccagtggc
15901 gcggcgactt ccagttcaac atcagccgct acagtcaaca gcaactgatg gaaaccagcc
15961 atcgccatct gctgcacgcg gaagaaggca catggctgaa tatcgacggt ttccatatgg
16021 ggattggtgg cgacgactcc tggagcccgt cagtatcggc ggaattccag ctgagcgccg
16081 gtcgctacca ttaccagttg gtctggtgtc aaaaataata ataaccgggc aggggggatc
16141 taagctctag ataagtaatg atcataatca gccatatcac atctgtagag gttttacttg
16201 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
16261 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
16321 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
16381 tatcttatca tgtctggatc cggaataact tcgtatagca tacattatac gaagttatgt
16441 ttaaacggcg cgccccggaa ttcgccttct gcaggagcgt acagaaccca gggccctggc
16501 acccgtgcag accctggccc accccacctg ggcgctcagt gcccaagaga tgtccacacc
16561 taggatgtcc cgcggtgggt ggggggcccg agagacgggc aggccggggg caggcctggc
16621 catgcggggc cgaaccgggc actgcccagc gtgggcgcgg gggccacggc gcgcgccccc
16681 agcccccggg cccagcaccc caaggcggcc aacgccaaaa ctctccctcc tcctcttcct
16741 caatctcgct ctcgctcttt ttttttttcg caaaaggagg ggagaggggg taaaaaaatg
16801 ctgcactgtg cggcgaagcc ggtgagtgag cggcgcgggg ccaatcagcg tgcgccgttc
16861 cgaaagttgc cttttatggc tcgagcggcc gcggcggcgc cctataaaac ccagcggcgc
16921 gacgcgccac caccgccgag accgcgtccg ccccgcgagc acagagcctc gcctttgccg
16981 atcctctaga gtcgagatcc gccgccacca tgattgaaca agatggattg cacgcaggtt
17041 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
17101 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
17161 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
17221 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
17281 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
17341 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
17401 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
17461 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
17521 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
17581 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
17641 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
17701 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
17761 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
17821 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
17881 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
17941 gcgcggggat ctcatgctgg agttcttcgc ccaccccccg gatctaagct ctagataagt
18001 aatgatcata atcagccata tcacatctgt agaggtttta cttgctttaa aaaacctccc
18061 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
18121 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
18181 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
18241 gatccggggg taccgcgtcg agaagttcct attccgaagt tcctattctc tagaaagtat
18301 aggaacttcg tcgagataac ttcgtatagc atacattata cgaagttatg tcgagatatc
18361 tagacccagc tttcttgtac aaagtggttg atatctctat agtcgcagta ggcggtctgg
18421 tgtagtcatt gagcaaagct agactctgac tgtcctttac agggaggaca gcaagccagc
18481 tgtccagatc ctcccaggca gtgtttacag ccccactagt gaggctgagc ttagagaaaa
18541 ggaaaggttt gggaaacttt tcttgaattt tctgtgtttc tcccagaatg tatttttttc
18601 tcaagacagg cctgtgtcct tcgcttcttt atctcttccc tcaaagagct ttattgatct
18661 gagcatccat atcaatcaat acagagtgaa cgtctgccct ctaggcacag tggcttcttg
18721 actacactag tgtttctctt ctctcctcac agattttgat aagcctgact tcaagagaag
18781 catagtgtgt ctggaagacc tgcaggttgg gacagttctt acaggcaaag tggagaatgc
18841 tactctgttt ggggtctttg tggatatagg agtggggaag gcggggctga ttcctatccg
18901 gttcataaca gaagccaagc tgtccaaaac aaagaggaga agaagcctgg gcctgggccc
18961 cggagagaaa gtggaggtga aagtcctcaa tgtcgacatt ccccgatctc ggatttcgct
19021 cgacctcctc cgggtgttgt gagtgcccca ccgaaggcca aacaccaatg tgttccctca
19081 cttctacagc ctgacaagga cgagccagag ctcatggaac ctagagagcg gggggggggg
19141 ggctcctggg tagaagtgtt catcacacta tcctgactcc cacggaaagc aacaggagac
19201 ctcgcatggt gtcctacagc agcagtttct gtctttgtaa ttccttgttg accacataca
19261 gcaggcagct tcacgtcctc ccgctaacat ctgctgcctt agacctgggt gggatgtctt
19321 gcttatttga aatgtgtagt gttgattgac acattaagac tagcagtctt taatcctgcc
19381 actttattga ggtgttatta gcacttttgt tcataattca taattttgta ataatcttta
19441 ttttccctta gcaaaccaag taaaaataaa ctagtaaaag attatcatgt tttatgacca
19501 tcaacggaat cattttaatg tgttcctcag aacgtatgta ttgacttcta caataaatga
19561 ctggctggtg actttggtgt gctgttcatt gagtgaatgc tagaagccac actggattaa
19621 agatgtgatt tgtggaactg tgccttgggg aagaggggag attgtggaca tttccagaat
19681 gactttaatt aacgtagacg taatttagag atggcgcaac gcaattaatg ataacttcgt
19741 atagcataca ttatacgaag ttatggtctg agctcgccat cagttcattt cttagccatc
19801 atctaagaag atagcgaccc tgactctatt cttgtttggt gggaaaggta agagttgcgt
19861 gacagctcag ggtcagagga ggtgagcaga gcccgcactg attactcagt gaagtgctca
19921 gggcccggac agtctccatg agtgatgtgt ggagggcgga tacaaaagtg tgctcagccc
19981 tatttctttg tcttgggcaa ttccttaatt tataagtaag cagccattac ccttgaagac
20041 ttaagtgctg ctgctgcttg gcctcaggat gcccaattgg ggatcatata atcacccagt
20101 gggagttata aggaagtagt gtactcgtgg tacacaccag ttccacctgg tggcttccta
20161 ttctcaaaac acttggccca tctaactagt ggacataggt gtcttgaagc cctgtttgcc
20221 tgtttcattc ctgcaagtct tttctgcttt ttactggtct ttgtctgaca atgagtgcta
20281 atgtgttctc agttggtctt tctactcatt cttgccaacg ctgtctggtt catattgtgc
20341 agatgaatgc taagtgatgt ccttgggtgc tcccaggagt gctcctcctc ctgggcactt
20401 tggatgaagg agcctgaata gctctatgcc cttgacagct aaggagcagg aggagaacca
20461 ggtcagtgtg ccctttgccc tttttatcta acatgataga aactctgaat agtccaacct
20521 gggaaaaaag ggccacatat ctgtcatcgc tgcatccctt gctgatgtgg gttttagagt
20581 tggagctggg tcggaggcag tgacattctg tagagtttgg ctgtcgagaa gagggggaaa
20641 tagcataata gctagacagt gaaatgtaac ctagagttcc tatgtgtttc tagagaaaag
20701 agataacctc taacaatgaa gagaaatttg atacaaagga tgggaaagtt actgaatgat
20761 atcctcaatt gaatagattc agctggaatc cagttcacag tggggacttt accgtaggga
20821 tgcagtgaca actggattgt cacttgtacc cattggaaga ggagaactca gctttctccc
20881 gtccagtgac actgagtaca ccagtgtgat ggtttgtata ttcttggacc agggagcggc
20941 accatctgaa agtgtggcct ttttggaata ggtgtgacct ggttggaatg ggtgtgtcac
21001 tgtgggtgtg ggtataagat cctcacccta gttgcctgga agtcagtctt ccactagcag
21061 cctttggatg aagatgtaga actctcagct ctgcctgcgc catgcctgga tactgccatg
21121 ctcctacctt gatgataatg gactgaacct ctgaacctgt aagccagccc caattaaacg
21181 ttgtttttta taagacttgc cttggtcatg gtgtctgttc acaacagtaa aaccctaact
21241 aagacaacca gccactctgg gacaggcctc ctgttcagga ggagttgacc aacataaagt
21301 gaacaatggg ctccacagga gtttttgtta ttgttttggt tttgtgtgtg agcttgtatt
21361 tggtaacagt ttgatacttg gggggggggg tgacaatggc tttattttgt tttcttcgtt
21421 ggggggcagt tgtattgagt ttttgtttga tttttgagaa agaacttaaa gttgggtaga
21481 tgaagaggag aagaatatga tcaaatatat ttaaatttta aaaattaaaa taatgtttat
21541 aaaagataag aatacaaaag ttcatccgca ttagtagaag aggagacagt atataagcac
21601 agaggtaaga gctcatatga gaacttacag aaggtctttg gatgtcttct ggtttcctta
21661 ttaaaatagg gaagaaagga tcagggggtt gagaagaaaa agacaaaaaa tggggtaaga
21721 tagactgaaa gctaggcctg tgctcagaca cggaggaagg gcagccagcc cgtgctcaga
21781 ggagggacgg tcagcccgtg ctcagacaga ggagggacag tcagcccatg ctcagacatg
21841 gaggaaggca tcatagtcag atttaccagc cagatcagct atggggctgc agggtcaggt
21901 caggagaaga gagacctgct tggatttgga agtatgccca acaagtacac cctagagaaa
21961 agggcagaaa cataaaggct tgtggataaa gaaactacta actgaaatgt agacttggta
22021 aaaagagaag tgggaaccca agggaaagaa ggggttggtg ggaggagcag gagaaactgc
22081 aggctaaagg attgcaggta gagccgagga actttgaagt gtggataaag ggcataaagg
22141 ttagagataa gatcaactga ggtgcataac cttcacagcc tggagtgcct cagatatggt
22201 aatgatgggg taagagagat gatgacatag cacataaata gaagatgaga cggtgaccac
22261 tagcagtcag caactgagaa tttgaaagcg tctgtagata aagagcttgt ctttgtggtt
22321 gcaaaggaac actgctctga aatacagtga cagaccactg gaggctggag aggttgtcta
22381 gtggttagga actcttgctg atcctccaga ggacccaagt tcagttccca gcaccctgta
22441 aattcagtgc caagggctcc agcaccctct tctggcctat atgagcacat gggacattca
22501 cccacacaaa tatagataca cacaaataga aatttaaaat ctgagaaaaa gcatggtaga
22561 aggggctcaa aagtcaagat tctggctata tttgcaagta agtaaagatg gtcccgaggc
22621 tcctgtcgta agtaactggg agcgtagaat tggcgtctac tgagatggag aaccatgaga
22681 ggggcagtga ggagtgtatg tagaacaggt gacattctag atgttcagta gagtatatcc
22741 aaggggacat gctaggttag gtagtgactt ggtttctttc cccgttgctg tgataaaaat
22801 actccaataa aagcgactta agggagaaag ggcttattgg gctcacatcc aggttgtagc
22861 ccatcatgac atggccaaga agtcataatg ttgggataga gagatctggt cacataacac
22921 ccacataaca gaactggtct ccagtcagga gagagtgatc agtgcatgct agcgtcactc
22981 cctgtcatcc atattgtcta ggattcagag cccagggaag gatgctgcca acagtggcaa
23041 ggtcccacat cctctgtcga cagaagagcc tcagctagac agtctcaccg agactctctt
23101 ccaagctaat tgtagattgg ttccagtgaa caattaaaac tgaccatcac agaaggaaca
23161 gttaactatt ttagactgaa gaccaggaga gaactctgga aaagccagtc atgagtccag
23221 aagagaccac tagtgaaata ggtgagcata aggaaaaaaa agagggaaag aaaccatggc
23281 agacagtctt aaaccctagt ctctccagag actgaaatgt atttcttgct gagcgagagt
23341 taactgaagg gaacaggagt agctaatgga gatggtcttg ccgggtggta ctggcagaat
23401 ggagatggtc ttgccgggtg gtactggcag aatggagatg gtcttgcagg gtggtacagg
23461 cagaattcac ccaccaagcc gcttctaaga aagttagaag agtgagtagg tcgggcatca
23521 gtgccaccac ctagattatc ttgttctctc tctctctctc tctctctctc tctctctctc
23581 tctctctctc tctctctctc tctctctctc tctctctctc tctgtgtgtg tgtgtgtgga
23641 gtgctggctg tgctagctgc agtgcagtgg tgatctcagt gtgcagtgct gggtttagcc
23701 tggccttggg gatccagagt agccctgcac tgcctcttat ggactgcaca ccatgtaagg
23761 aaaaagaaaa gtcatttatt taaactgggg ataaaggcca ttccctactg catgtcactg
23821 ttcccagatg tatccttttc atgaagaaaa ttgcctcaca tacagtaggt gttcaataaa
23881 agtcaatgaa tg
//