LOCUS KO-first_condition_ready_75715:82078_MGI:1914298 23282 bp dna circular UNK DEFINITION Mus musculus targeted KO-first, conditional ready, lacZ-tagged mutant vector Vma21 targeting project(s): 75715:82078 ACCESSION unknown SOURCE Mus musculus ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus. COMMENT cassette: L1L2_Bact_P COMMENT design_id: 55734 COMMENT backbone: R3R4_pBR_DTA+_Bsd_amp FEATURES Location/Qualifiers misc_feature 29..152 /note="R4 gateway site" promoter 399..911 /note="PGK promoter" CDS 925..1581 /note="DTA" misc_feature 1706..1959 /note="BGH pA" promoter 2034..2100 /note="EM7 promoter" CDS 2101..2499 /note="BsdR" CDS complement(4193..5053) /note="AmpR" misc_feature 5272..5395 /note="R3 gateway site" misc_feature 5401..5408 /note="AsiSI" misc_feature 1..5433 /note="Synthetic Backbone" exon 7363..7545 /db_xref="ENSEMBL:ENSMUSE00000699822" /rank="1" /note="ENSMUSE00000699822" misc_feature 5434..10233 /note="5 arm" misc_feature 10261..10285 /note="B1 Gateway" misc_feature 10298..10345 /note="Frt" intron 10346..11220 /note="mouse En2 intron" misc_feature 11218..11220 /note="Splice Acceptor" exon 11221..11393 /note="mouse En2 exon" misc_feature 11405..11998 /note="ECMV IRES" gene 11999..15059 /gene="lacZ" /note="lacZ" misc_feature 15098..15341 /note="SV40 polyadenylation site" misc_feature 15345..15378 /note="loxP" promoter 15405..15924 /note="human beta actin promoter" gene 15950..16744 /gene="NeoR" /note="NeoR" misc_feature 16944..17185 /note="SV40 polyadenylation site" misc_feature 17202..17249 /note="Frt" misc_feature 17256..17289 /note="loxP" misc_feature 11235..11312 /note="FCHK std_gpr" misc_feature 17305..17325 /note="B2 Gateway" misc_feature 10234..17355 /note="Synthetic Cassette" exon 17743..17852 /db_xref="ENSEMBL:ENSMUSE00001296602" /type="targeted" /rank="2" /note="target exon 1 ENSMUSE00001296602" misc_feature 17356..18020 /note="Critical Region" misc_feature 18044..18077 /note="Downstream LoxP" misc_feature 18021..18100 /note="synthetic loxP region" exon 18506..22407 /db_xref="ENSEMBL:ENSMUSE00000699823" /rank="3" /note="ENSMUSE00000699823" misc_feature 18101..23282 /note="3 arm" ORIGIN 1 ccactggccg tcgttttaca ttaattaaca actttgtata gaaaagttga acgagaaacg 61 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa 121 tactgtaaaa cacaacatat ccagtcacta tggcggccgc aatcgggcag cgttgggtcc 181 tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg ctggcggggt 241 tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc 301 tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa 361 agtctggaaa cgggccgcct agcgatagct gtttatcgaa ttctaccggg taggggaggc 421 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta 481 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc 541 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc 601 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct 661 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca 721 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg 781 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc 841 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg 901 cctttcgacc tgcaggtcct cgccatggat cctgatgatg ttgttgattc ttctaaatct 961 tttgtgatgg aaaacttttc ttcgtaccac gggactaaac ctggttatgt agattccatt 1021 caaaaaggta tacaaaagcc aaaatctggt acacaaggaa attatgacga tgattggaaa 1081 gggttttata gtaccgacaa taaatacgac gctgcgggat actctgtaga taatgaaaac 1141 ccgctctctg gaaaagctgg aggcgtggtc aaagtgacgt atccaggact gacgaaggtt 1201 ctcgcactaa aagtggataa tgccgaaact attaagaaag agttaggttt aagtctcact 1261 gaaccgttga tggagcaagt cggaacggaa gagtttatca aaaggttcgg tgatggtgct 1321 tcgcgtgtag tgctcagcct tcccttcgct gaggggagtt ctagcgttga atatattaat 1381 aactgggaac aggcgaaagc gttaagcgta gaacttgaga ttaattttga aacccgtgga 1441 aaacgtggcc aagatgcgat gtatgagtat atggctcaag cctgtgcagg aaatcgtgtc 1501 aggcgatctc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc 1561 tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact 1621 actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca 1681 gtggtggaat gcagatccta gagctcgctg atcagcctcg actgtgcctt ctagttgcca 1741 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac 1801 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat 1861 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca 1921 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgag 1981 atccactagt tctagcctcg aggctagagc ggccgagttt atcctcgagc acgtgttgac 2041 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc 2101 atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc 2161 aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc 2221 cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg 2281 gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga 2341 aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg 2401 catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt 2461 cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag cacttcgtgg ccgaggagca 2521 ggactgagaa ttccccggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca 2581 ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga 2641 ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa 2701 cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt 2761 ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc 2821 aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt 2881 ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac 2941 gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac 3001 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc 3061 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc 3121 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg 3181 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc 3241 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 3301 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 3361 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 3421 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 3481 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 3541 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 3601 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 3661 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 3721 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 3781 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 3841 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc 3901 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 3961 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 4021 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 4081 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 4141 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 4201 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 4261 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 4321 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 4381 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 4441 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 4501 ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 4561 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 4621 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 4681 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 4741 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 4801 cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 4861 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 4921 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 4981 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 5041 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 5101 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 5161 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct 5221 ataaaaatag gcgtatcacg aggccctttc gtcttcaaga aaattgtcga ccatagtgac 5281 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat 5341 atattgatat ttatatcatt ttacgtttct cgttcaactt tattatacaa agttgcggac 5401 gcgatcgccg gcggataaca atttcacaca ggacacctcc atcttagaca tctaggagtg 5461 ggaaatgatt aacatgttgt ttaaacaaca gtccaggttg tggtcctttg ttatcgtgat 5521 cttggcaaag attaatacat ctacattgtt tttgcggcca caactcagtt gtttaacata 5581 tattgtctaa aattgggtac atacaatcat tcttttcaca ttcatatttt aaagatttat 5641 tctatcaagt atactacagc atattttagg ttttagtaat ggtctttcca tttaccctgt 5701 agatgaacag gattactttg tcagtgtgac aaaatttatg cctcaagtca aataatgtga 5761 tgttttagtt gttgaagttc attagatttt tagaactaac aaaacttgtt ctcagaatac 5821 ccattattaa gtttatgggt tctccggggg tttaaggacc atggggtgct aagcaagttc 5881 aggaatcagg aaagtgttcc ggcaaagagc cctacctcat catgtctggt ggtatagatc 5941 tggaatccca gccacctggg aggctgagac agaagaattg agtccaaaac ctgctaggct 6001 gtggactgag ttcaaggctg gcttgagtga gtgagaccct atgtcaaatt ttaaaagggg 6061 atggggagtt ctgggagtag ttgttcagta gcagagcaca catccagcat gctacagaga 6121 tcctggtttc catctccatc acccctccat gccctctccc tccctcctcc ccctccccct 6181 ctcttgcttt cactccaaat caacatagac aataatttag gatttatgga acatgctact 6241 tatgttgcat ctacccaata ttaaagttgt agagacaatg caaatgaata tgcatgactc 6301 agttccaaac gttctaatta tgaatattaa aattcaaatg gcttttctga gtcgtttctg 6361 agatctttga cattttctta cccctcagaa gagagaaaac ttggctacag gccccatcta 6421 tttgactggc tgcccagtgt gtcacgtgga acacccagtt ttccacgacc tgagttcata 6481 cattaggttc acctaaggat ctgcatgagg cctagagagg ggttactcca tttcaactga 6541 aacgcaagct tcctactgct ccatcaaggc tggcttactt gtcctttcag atgtgcttag 6601 tatcccgtgg tgcagaaaat aggtatgtgg tcagcagccc tttccgggct tgaccattta 6661 agggctgtca cagtattagc agccaaaggc aacctaattt tgttaagcct gtcctagagc 6721 ttacacaccc ttttctgttg gtgcctctcg ggttgcctgg gagcttaggc ccatctgtcc 6781 ttactatttt gagtccctgt tgtctcattg gcggctttct gaacagctgc aaaacatact 6841 tttaacacta agtggaagtt tgggtcaccg gctcccactg tggaacagtc actctcagca 6901 agggctttca gcgagagttc tctagtttta ctgactccgg tgagtgttaa actaacttct 6961 ctcaactcct tgctagattg ccaaggcggg taggaatgaa tgaggtcttt gcttatgggc 7021 tggtggaata atgtgcactg ctttttgtgt tatcggaaac tgctcggttg cctagcaccg 7081 cagccgacag ctgggtcgtc ctttccgcgg ctcctctacc tcgggtcacc aggctcccgt 7141 gacaccgcaa gcgggaccga gaggcgaacg acaggcactg ggcccggaag gacaatgctg 7201 cgcggaaagt cccggctcaa cgtggagtgg ctaggctact cgcccggcct gctcctagag 7261 aatgatccgc tggcagcggg acggacacca cgaacccccc gcgggtaagt cacgtgagtg 7321 cttgcagcgt acccgcgccc gcgcgcgctg cccgcgcgtc actacgccgc gccacgcgag 7381 cgtgcttcgg caggggcact tccggcgcga ccggcctaga ctgtcaccct gttagccgcc 7441 gctgagcctc cgctaactac ccggcgcctg ctgctgccct cccggccacg ccatggagcg 7501 tcttgataaa gcagcgctca atgcgctgca gcctccagag ttcaggtatc cctgagttcc 7561 gctgggcaac ggtggccgct cggctctggc ctggcgctgg ccccagggca ggcaggaggc 7621 agggtgggct tggggacgac ccctccacgg gtcaccgcag gctgatgaag aggtaaagta 7681 gtttggagcc tgcagcttct gagtggcggg ccctcagttc acgcttcctt gctcttcccc 7741 tccctgtttg gctgctttgc agtcttcccc tttgccaggt ggctcttttg aggataggcg 7801 cccggtctcc tttatcttgg cgcttgcgag cccctacatc acatggtttt ttgtaaatat 7861 ctagtgtgtg aatacagtgg aggcaggctc tgatgtgagg tcacttctct tgaggggaat 7921 caattagtag gataaggact ggtgactgaa cagaaagaat gtgatagtag gcctctctga 7981 ggcaagggcc tggggaagaa gttagcagcc attaattgac atcatcagat ttttcttaat 8041 tcttagtttg gctgactact taaattttaa cttgagaaga aaaaggaagt tgaattcaca 8101 taagtgaatt ttgtgatcca agatcacaaa attagaccgc tgacaggtct cagcagcccc 8161 tagtcaggta taaaataagt caaccaaatg acattcattg ctttgtttta tgtcactgat 8221 actacctgac ctgatttgta ccagcagaac caacaggtaa agtgagctat gactagaaac 8281 taagaagaaa atggtctaac tgctagggga aggtgggtaa ctgcttttct tgtaagtttt 8341 ttgcacactt aactttgact atcactcata ggctttggat attttaggtg gatattttga 8401 tcagtaacag actgcaaatg tcttccatgg gattgtatta tctagtgata tatcagccat 8461 tttagtttgt atggatatgc tccgggatgt ttgtggagtg gcagaacctt ctgaaaatgc 8521 atttctcaaa atatttcact atttttaaat gggggataac tacttaaact actgaagtta 8581 taaaagagaa aagtcatttt tttaaaaagt ttaagttaga gaagagtaag aatgtattga 8641 aaataataga ccataaatta ataacatatg aacatacagg gtgtggggac ctggatgtta 8701 atatatagaa aagattatat ttacacgatg gaactatggt tttgttcaca ctaatatttt 8761 tattatgttg tatcatttta ataagtgttt ataaaattat ttttaagctt tattgtttta 8821 aattgtgtgt gtgtgcatgt gcgtgcatgt gtgtatgtgt gtgtgtgtgt gtgtgtgtgt 8881 gtgtgtgtga ttcaggttca ttaaggaggt cagaggcatt gttggatcct ctgaatccga 8941 agttaaagca gttgtgagct gcctgacatc agtgctggga atggaacttg ggttctctgg 9001 aagaaaagta tgactgagcc atctctctgg cccatccttg taaaaattat accagatagt 9061 agatacttaa gagagataat ttaaaaaaca cactagttgt agtggcatag acctcttaaa 9121 actacctatt gggaaatccg aggcagaagg atcatcattt caaggcctgc ttaggctaca 9181 gaatgagttc taggctaaca tgggcaactt aatgaaattc agtctctaaa ggtaaataag 9241 aagagcttga gaagtagcca catggtagag catttgccta acatatatgt agctcagtaa 9301 tggtagaata tagtagaagg taatacattt aggtaccaag agataatctt agaatgtttt 9361 cttctaccag tgtactgccc atgacaatca atataagagt aagattttat ttctcccact 9421 tatacaagga ctgattaacc cagaaaactt gctaatacaa tgtagtttta tgagatataa 9481 ttttcatgta tattagctgt ttaaacttaa tagccttgtg tctgctttat tgtaatttga 9541 catacagttc catattttct gcccctatcc atagttgcca gtgtttattt tttctataga 9601 tttggtcatt tttaatatat atatacatac atacacacat acatacatat ataattgtgg 9661 aatatgacta ccttgattgt ttgcgcatat atacacccac cctaacacac atttcactgt 9721 ctctctcttt cacacacaca tacaaattaa gaagtggctg cgtctgtcta gaatacttag 9781 ccatgactgg agtgtctaga cggacttttg tttgttgatg gaacttttta ctgtcagatt 9841 tttcctcttc cctgtctatc agaatagttt aaaatacatt tctattttct aggattttgg 9901 ttgatttttc catcacactt ttaaaaagta atcacattaa aggaaacaca attgtttaaa 9961 tgatgaagta ttccaaaaag aatgccttgt tttttgtgaa aatgcatttc tctgcaattt 10021 cagactagct tcaaattatg caaactaagc agtaatgcta actatgggtt ttacatctag 10081 tctttaaaaa acactgtgtt ctataccctt cctatcatca tactttggct caaaccagag 10141 gactatacca gaaataaacc agaaaagaca gatgtattct agaatcaaac tgattctatt 10201 ttcagttgtt taaatgatga aatattcaaa aagaaggcgc ataacgatac cacgatatca 10261 acaagtttgt acaaaaaagc aggctggcgc cggaaccgaa gttcctattc cgaagttcct 10321 attctctaga aagtatagga acttcgaacc ctttcccaca ccaccctcca cacttgcccc 10381 aaacactgcc aactatgtag gaggaagggg ttgggactaa cagaagaacc cgttgtgggg 10441 aagctgttgg gagggtcact ttatgttctt gcccaaggtc agttgggtgg cctgcttctg 10501 atgaggtggt cccaaggtct ggggtagaag gtgagaggga caggccacca aggtcagccc 10561 ccccccccta tcccatagga gccaggtccc tctcctggac aggaagactg aaggggagat 10621 gccagagact cagtgaagcc tggggtaccc tattggagtc cttcaaggaa acaaacttgg 10681 cctcaccagg cctcagcctt ggctcctcct gggaactcta ctgcccttgg gatccccttg 10741 tagttgtggg ttacatagga agggggacgg gattcccctt gactggctag cctactcttt 10801 tcttcagtct tctccatctc ctctcacctg tctctcgacc ctttccctag gatagacttg 10861 gaaaaagata aggggagaaa acaaatgcaa acgaggccag aaagattttg gctgggcatt 10921 ccttccgcta gcttttattg ggatccccta gtttgtgata ggccttttag ctacatctgc 10981 caatccatct cattttcaca cacacacaca ccactttcct tctggtcagt gggcacatgt 11041 ccagcctcaa gtttatatca ccacccccaa tgcccaacac ttgtatggcc ttgggcgggt 11101 catccccccc cccaccccca gtatctgcaa cctcaagcta gcttgggtgc gttggttgtg 11161 gataagtagc tagactccag caaccagtaa cctctgccct ttctcctcca tgacaaccag 11221 gtcccaggtc ccgaaaacca aagaagaaga accctaacaa agaggacaag cggcctcgca 11281 cagccttcac tgctgagcag ctccagaggc tcaaggctga gtttcagacc aacaggtacc 11341 tgacagagca gcggcgccag agtctggcac aggagctcgg tacccggaag atctggactc 11401 tagagaattc cgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga 11461 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa 11521 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc 11581 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc 11641 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg 11701 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca 11761 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag 11821 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct 11881 ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaacg tctaggcccc 11941 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataagcttg ccacaaccat 12001 ggaagatccc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa 12061 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga 12121 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc 12181 accagaagcg gtgccggaaa gctggctgga gtgcgatctt cctgaggccg atactgtcgt 12241 cgtcccctca aactggcaga tgcacggtta cgatgcgccc atctacacca acgtgaccta 12301 tcccattacg gtcaatccgc cgtttgttcc cacggagaat ccgacgggtt gttactcgct 12361 cacatttaat gttgatgaaa gctggctaca ggaaggccag acgcgaatta tttttgatgg 12421 cgttaactcg gcgtttcatc tgtggtgcaa cgggcgctgg gtcggttacg gccaggacag 12481 tcgtttgccg tctgaatttg acctgagcgc atttttacgc gccggagaaa accgcctcgc 12541 ggtgatggtg ctgcgctgga gtgacggcag ttatctggaa gatcaggata tgtggcggat 12601 gagcggcatt ttccgtgacg tctcgttgct gcataaaccg actacacaaa tcagcgattt 12661 ccatgttgcc actcgcttta atgatgattt cagccgcgct gtactggagg ctgaagttca 12721 gatgtgcggc gagttgcgtg actacctacg ggtaacagtt tctttatggc agggtgaaac 12781 gcaggtcgcc agcggcaccg cgcctttcgg cggtgaaatt atcgatgagc gtggtggtta 12841 tgccgatcgc gtcacactac gtctgaacgt cgaaaacccg aaactgtgga gcgccgaaat 12901 cccgaatctc tatcgtgcgg tggttgaact gcacaccgcc gacggcacgc tgattgaagc 12961 agaagcctgc gatgtcggtt tccgcgaggt gcggattgaa aatggtctgc tgctgctgaa 13021 cggcaagccg ttgctgattc gaggcgttaa ccgtcacgag catcatcctc tgcatggtca 13081 ggtcatggat gagcagacga tggtgcagga tatcctgctg atgaagcaga acaactttaa 13141 cgccgtgcgc tgttcgcatt atccgaacca tccgctgtgg tacacgctgt gcgaccgcta 13201 cggcctgtat gtggtggatg aagccaatat tgaaacccac ggcatggtgc caatgaatcg 13261 tctgaccgat gatccgcgct ggctaccggc gatgagcgaa cgcgtaacgc gaatggtgca 13321 gcgcgatcgt aatcacccga gtgtgatcat ctggtcgctg gggaatgaat caggccacgg 13381 cgctaatcac gacgcgctgt atcgctggat caaatctgtc gatccttccc gcccggtgca 13441 gtatgaaggc ggcggagccg acaccacggc caccgatatt atttgcccga tgtacgcgcg 13501 cgtggatgaa gaccagccct tcccggctgt gccgaaatgg tccatcaaaa aatggctttc 13561 gctacctgga gagacgcgcc cgctgatcct ttgcgaatac gcccacgcga tgggtaacag 13621 tcttggcggt ttcgctaaat actggcaggc gtttcgtcag tatccccgtt tacagggcgg 13681 cttcgtctgg gactgggtgg atcagtcgct gattaaatat gatgaaaacg gcaacccgtg 13741 gtcggcttac ggcggtgatt ttggcgatac gccgaacgat cgccagttct gtatgaacgg 13801 tctggtcttt gccgaccgca cgccgcatcc agcgctgacg gaagcaaaac accagcagca 13861 gtttttccag ttccgtttat ccgggcaaac catcgaagtg accagcgaat acctgttccg 13921 tcatagcgat aacgagctcc tgcactggat ggtggcgctg gatggtaagc cgctggcaag 13981 cggtgaagtg cctctggatg tcgctccaca aggtaaacag ttgattgaac tgcctgaact 14041 accgcagccg gagagcgccg ggcaactctg gctcacagta cgcgtagtgc aaccgaacgc 14101 gaccgcatgg tcagaagccg ggcacatcag cgcctggcag cagtggcgtc tggcggaaaa 14161 cctcagtgtg acgctccccg ccgcgtccca cgccatcccg catctgacca ccagcgaaat 14221 ggatttttgc atcgagctgg gtaataagcg ttggcaattt aaccgccagt caggctttct 14281 ttcacagatg tggattggcg ataaaaaaca actgctgacg ccgctgcgcg atcagttcac 14341 ccgtgcaccg ctggataacg acattggcgt aagtgaagcg acccgcattg accctaacgc 14401 ctgggtcgaa cgctggaagg cggcgggcca ttaccaggcc gaagcagcgt tgttgcagtg 14461 cacggcagat acacttgctg atgcggtgct gattacgacc gctcacgcgt ggcagcatca 14521 ggggaaaacc ttatttatca gccggaaaac ctaccggatt gatggtagtg gtcaaatggc 14581 gattaccgtt gatgttgaag tggcgagcga tacaccgcat ccggcgcgga ttggcctgaa 14641 ctgccagctg gcgcaggtag cagagcgggt aaactggctc ggattagggc cgcaagaaaa 14701 ctatcccgac cgccttactg ccgcctgttt tgaccgctgg gatctgccat tgtcagacat 14761 gtataccccg tacgtcttcc cgagcgaaaa cggtctgcgc tgcgggacgc gcgaattgaa 14821 ttatggccca caccagtggc gcggcgactt ccagttcaac atcagccgct acagtcaaca 14881 gcaactgatg gaaaccagcc atcgccatct gctgcacgcg gaagaaggca catggctgaa 14941 tatcgacggt ttccatatgg ggattggtgg cgacgactcc tggagcccgt cagtatcggc 15001 ggaattccag ctgagcgccg gtcgctacca ttaccagttg gtctggtgtc aaaaataata 15061 ataaccgggc aggggggatc taagctctag ataagtaatg atcataatca gccatatcac 15121 atctgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca 15181 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata 15241 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 15301 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cggaataact tcgtatagca 15361 tacattatac gaagttatgt ttaaacggcg cgccccggaa ttcgccttct gcaggagcgt 15421 acagaaccca gggccctggc acccgtgcag accctggccc accccacctg ggcgctcagt 15481 gcccaagaga tgtccacacc taggatgtcc cgcggtgggt ggggggcccg agagacgggc 15541 aggccggggg caggcctggc catgcggggc cgaaccgggc actgcccagc gtgggcgcgg 15601 gggccacggc gcgcgccccc agcccccggg cccagcaccc caaggcggcc aacgccaaaa 15661 ctctccctcc tcctcttcct caatctcgct ctcgctcttt ttttttttcg caaaaggagg 15721 ggagaggggg taaaaaaatg ctgcactgtg cggcgaagcc ggtgagtgag cggcgcgggg 15781 ccaatcagcg tgcgccgttc cgaaagttgc cttttatggc tcgagcggcc gcggcggcgc 15841 cctataaaac ccagcggcgc gacgcgccac caccgccgag accgcgtccg ccccgcgagc 15901 acagagcctc gcctttgccg atcctctaga gtcgagatcc gccgccacca tgattgaaca 15961 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg 16021 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 16081 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc 16141 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt 16201 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 16261 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 16321 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 16381 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg 16441 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct 16501 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 16561 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc 16621 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta 16681 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 16741 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga 16801 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac 16861 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccccg 16921 gatctaagct ctagataagt aatgatcata atcagccata tcacatctgt agaggtttta 16981 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt 17041 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 17101 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 17161 aatgtatctt atcatgtctg gatccggggg taccgcgtcg agaagttcct attccgaagt 17221 tcctattctc tagaaagtat aggaacttcg tcgagataac ttcgtatagc atacattata 17281 cgaagttatg tcgagatatc tagacccagc tttcttgtac aaagtggttg atatctctat 17341 agtcgcagta ggcggggaat agccattaat tgtcttcttg gtttactctc accctgctgt 17401 atgttctatg aagccctaac taggtgttga atcagtttga ttctagaata catctgtctt 17461 ttctgcttta tttctggtat agtcctctgg tttgaacaga agtgtgtata gtccttgggg 17521 aggagctgtt tgattgcaaa tgggtttggg tttttttgat ataatgttta gaggcttgtg 17581 tgcctgcata tcaatctcag gatctgactt gggtgcacac atgcgcatgc ttgtctcttt 17641 gccgcgttct ggattgcaga agctatttgc tcagaattct tttatcttca ggatttttgt 17701 atttggatat ggtttgtgaa aattgtgttc actttcttcc agaaatgaaa actctttagc 17761 tgcaaccctg aagacactgc tgttcttcac agctttaatg atcacggttc ctatcggctt 17821 gtattttaca acgaaagctt atatatttga aggtaatctt tacgtacttt aagatatttt 17881 tattttctaa tttattctga gcttttatga gtccttttat tgtaaaactt gttttcttct 17941 tccttttgtg taagttaaat atgtggccaa tgacagtttg tatgctttgg ttggctgggt 18001 gtggtgctgt atgcctgtaa gagatggcgc aacgcaatta atgataactt cgtatagcat 18061 acattatacg aagttatggt ctgagctcgc catcagttca tggaggatat ttattactta 18121 cactgtattg ctttaaatgt tttgcacaaa ttcacctaaa gaatccccac aacaatactc 18181 tgaattaatt agaagcatgg tacaaaaagt gaataattgc aatctgcact accagtggaa 18241 atgggcgaag aaaagagaag agttggtaat tagtaattcc gacttttgtg tcatgagcac 18301 tgtccttcca tatggcctgc tttgctctct gccttagggt acaggagttt tgttttgttc 18361 ctgtatactt tggaactggg ctccacgact tggttgtagt tttctgtaat ggtgttcatc 18421 acaaacagaa ttcttaggtt ttgaattttg tagtttggta agtaataagt gtgtaataaa 18481 atgaaatatt ctcttttctt gataggtgcc cttggaatgt ctaatagaga cagctatttt 18541 tatgccgcaa ttgttgctgt tgttgctgtt catgtggttc tggccctgtt tgtctatgtg 18601 gcctggaatg aaggctcacg acagtggcgt gaaggcaaac aggattaaag tgacatcacc 18661 ttttgatagc atgaaattta tttttgaata tgttacataa ttccggggta ttgataataa 18721 atacatttgt ttgaataaag ttgaaagaac atgttaaaat cagtcttaaa gagtcagtca 18781 catttggcta aattttgacc cacccaataa tatggttact ttgaactttg aatctccatt 18841 tgcaaatgaa aatgtggaaa agttggagag gtacagatgt atatgtttca tgagttatct 18901 cttatcctag ggagaattta catatcttac aataacaaaa atgatagcaa tgtggcttat 18961 acttgctttt cagttttatt tatttgggta tattaagaaa tacttcatga agcaaatcta 19021 aattcccttt tttactgaag gacttcgctg gaattaccag gctcttgggt ttgagattct 19081 actttcctta aaagttactt acattgtagg cattttagtt aatctgactt ttaagtcagt 19141 gaggaaagat tgctctcaca ttccattatt tcacagggaa agtcactgaa accacactat 19201 tcatgcaaaa gggtgtaggg ttactgagga cagccaactg ggctgactgc cagcattgtg 19261 aatatcacac tatcattgtg ccctcacttt gctttgaaat taggtttcag ttgcaaagtt 19321 tatcgaaatt ccacttaatt tgttgttcta ggttaaaata gttttagaat accaaaatgt 19381 tcagcattta caaattgaga gtctatacag gcttgaaaca tcacaattct tatgaggtgt 19441 tcatgaagtt gttttgtcta ttaatccttt ttttaaatga catctcagct acatcctgag 19501 cataagctat gttctaagat aatccttaga gtcttttttt gattagaaca accctgcgcc 19561 tgaaaaaaag cccagttccc ttggtacctt cagtctctgg tcagacagac cttttggtac 19621 tgtgtatgtg tataaaaacg acgaccccca aatatttatt tctgattaaa agataatata 19681 ttctggaaaa cactggaaaa tacatataaa aattaaaaaa gcaaaaaatt tcccataatg 19741 ttaactacct gcagattatc agtagtttag ttcacaaccc ctgtacaagt acactaaaac 19801 cttgttttaa atttagatct tgtcaacttc accaaagagc tgtttgcagt ttttcagatg 19861 tgtggtaatt gcccattgcc ttacaaagta tatactatct ttattatttt aaaattccca 19921 tttcaatgtt ttcaccataa agaaatgtaa aaatttgagg tgataaccta gtttaaatac 19981 tatataacat gcatatctca aaacattaca tggcacccat aagcgtgtat gcttcttgtt 20041 tttatgttaa ttaaattgaa ttgtaaaaat gaagaaattt caaatacctg ttactgataa 20101 tcctaatata ataaatggcc cctgcttaaa tatttgctat tattgatttg atggtctact 20161 gttattttca tgctgtggaa ctagactaga aaaataggtg attggatata aaaatatgtg 20221 cttgtgttat gttattgtta taaacaacaa gattattgtg attttaaaaa gattgttctt 20281 aattcttaaa tttaacagtt gtgtgtagtt taaatatatt ttaaaatgag cattctaacc 20341 atgagagtct ttattttgta aagactgcag tttttagtat tatgatattt gagtcctaga 20401 cattgtgtgc tagagttcta gagggttggt tcccttatta ggtggcctat tatatttaga 20461 attgtccact cagtttcttt ttatgtaatg ccaaggcttt tttcttatac tttaggaata 20521 ttgtactatt tgtaaaatat actgttattt tgttttattt caaacgtttg ctaactaatg 20581 gtacaggtct tttaaaaagt attttaccca gggatacatg taaacacatg taattttccc 20641 ttggcaataa agtacaatgt gtttgaccat taaaatattc acactggtat ttagaattat 20701 tataaaactt gttttaaagc attttttcta ggattttgac tgcttactta tgagtttgct 20761 gctgttaggt aacatttttt taaggttttt aactagtttc ctctttcaca aagtacatat 20821 ttcatgctgt gttagaagaa aagctacagt taacctgtta agtgttttcc ggcggaaact 20881 gttcactgat ggtcaaaacc tgattgtaat ggtgatccca gttagggagt ataacttgaa 20941 gtgtccttgc aagctactaa tttaacccca cttgctgtaa cagtactttt cccaacttct 21001 gatatgcctt ccgaataatc ctttcagtgc taaaaaaatg gaaggctgct gtaggatgca 21061 tctggtatca ctgactcttt aaatggatgc catttcacag gttagagtag cattctggtt 21121 ttcaatgata gtaaagaatt ttaaacctgc tattcaaagt aactgcgtat ttcatgggaa 21181 tctaatagta actataactt gaagtttaca tgagaaaagt catagtgaac cggttatggt 21241 gttccattat tttaggaagc agatgtagga ggattaggag tttaagacca ccctggactt 21301 catgagaccc tatatcaaaa gaacagcagc aacaacaaaa gctacaaaaa caatttgact 21361 taataataat aaaaaaaagc aactaatggg cttgtgctta ttttcatata aatcttttgt 21421 agacaggaca ttcaaatggg taagtgaaag tatacaagga agatgtaacc aaggaaggct 21481 gatgctggag gtgtgcgaaa gaaaggcttg agatccgttt tacatatatc cagtgtatct 21541 ccagacagtt tgtatttagt gtgtaagtat agtaaatctg tagacaaagt gttcagcgat 21601 ggtacagtat ttgtcacttg tcttcagtta atagcaggtt tctttactac gttattcact 21661 agtgaaacac acagctattc agattttgct cctaaaattt atttttaaca gacttgtgct 21721 atagtgtagc ttgacaacat tttttatccc aggagaatgg agttacctta aaataaatta 21781 ctttcagaga tactggaaat ttactcatca atgcataacc tgtgaatttc ttaactccat 21841 tcctttacag acgtctagaa tctttccata tcgctgccgg ccacactttt aaacctgtgt 21901 taattgctat gtgtgcttga agtatctgtt cttttaaaga tgttctttaa gtcatattgt 21961 cagttttaca atctagtctt ccttccatgt ttggtgaaat ctcactgaac aggaataata 22021 gcaataacta acaacatctg cacagcacct tacagtttgc agagaacgtt cacatattct 22081 tgtctgagtt ttgcatagtg aacatgtcac agagatgtcc cttgatgtca atgctaagtg 22141 ttagaatgtt cagtcacagt gtcactggcc ttgctgcggc actgattagc acactgacgg 22201 gtggggggag gggggcgtgc cgatgtgctg tctgtaaagc tgcgtcatca caacggggcc 22261 tggatagctt agccacttgc ttcagactga cttgttaagt gatgcttttc tactctacct 22321 gaatggcttt tgtagtcagt atattgtagc ctgtaattta tattaattta ccatttgttg 22381 tcattaaaaa aagtgtctgt aatgctactg tgctgtgtcc tcattcggtg aatattgtta 22441 cattaacctc tggtttttaa aaaaatcttt gtgagcttgt ttctgcttat actgtagaat 22501 atgtccagat gtcagttttt aaatgtgtgt ttgcttctct gtgtgtcata tagcataagt 22561 gtagagctgt caaggaggtt tagtatatcc catgtaggta gagtggagat atttggggtg 22621 tgttggggta ctgatgtaga aggactggtt atattccaag taggtggacc agttagaact 22681 gatatggggt tagggtaagg aaagcttgcc tcagtcaagt ggaattttac acacctataa 22741 cctcagcagg tgaaggaaga gcagatgttc aacttcatcc tgagctgcat aagaagtatg 22801 aggcctattc gtgctacatt tctgtttgaa aacacaagtt atcttgtagg ttttggggag 22861 taaatagatt taaaatacca ctaaaggaat aaggttgaaa atacatgaga cttattaaag 22921 gtgagaagag aattactgga gaccttcagc atgttcggca ttacacacat gcacacacgc 22981 gcgcgcgcgc acacacacac acacacgtgc ctttgactag ttttctgcat cttgtttatt 23041 gcactggctg cagctccttt tggtgctccc gaggactcca gctaattctt agtaataaat 23101 accaccccat tccagctacc atttgaaatg tctcagtttc aaaagtatag ataatgagct 23161 gtccagggag ctcaggagac cgacaggtaa tagcactgta gaaaccaaag cgtgctgctt 23221 ctatggtgac tggaactcga ctgtggaatg gaattaaaaa taaccaattg tgcaaatgta 23281 ac //